"A straightforward stable isotope dilution analysis (SIDA) for the reliable quantitative determination of N-beta-C-18:0- to N-beta-C-24:0-alkanoyl-5-hydroxytryptamides (C5HTs) in coffee powder and beverages by means of LC-MS/MS was developed. The developed SIDA showing accuracy values of 92.6-107% and precision between 0.5 and 7% relative standard deviation for the individual derivatives allowed the sensitive and selective quantification of the target compounds in coffee beverages," investigators in Vienna, Austria report.
"Depending on the type of coffee, quantitation revealed C5HT levels between 65 and 144 mu g/L in filtered coffee and up to 3500 mu g/L in a French press …
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